Institutional Policies and Guidelines on Adjuvants and Antibody Production.

Pollard and J. M. Walker, eds. Clifton, N.J.: Humana Press. Reichmann, L., M. Clark, H. Waldmann, and G. Winter. 1988. Reshaping human antibodies for therapy. Nature 332:323-327. Reiter, Y., U. Brinkmann, K. O. Webber, SH. Jung, B. Lee, and I. Pastan. 1994. Engineering interchain disulfide bonds into conserved framework regions of Fv fragments: Improved biochemical characteristics of recombinant immunotoxins containing disulfide-stabilized Fv. Protein Eng. 7(5):667-704. Roberts, S., J. C. Cheetham, and A. R. Rees. 1987. Generation of an antibody with enhanced affinity and specificity for its antigen by protein engineering. Nature 328:731-734. Roberts, V. A., E. D. Getzoff, and J. A. Tainer. 1993. Structural basis of antigenic cross-reactivity. Pp. 31-53 in Structure of Antigens, Vol. 2. M. H. V. van Regenmortel, ed. Boca Raton, Fla.: CRC Press. Roberts, V. A., J. Stewart, S. J. Benkovic, and E. D. Getzoff. 1994. Catalytic antibody model and mutagenesis implicate arginine in transitionstate stabilization. J. Mol. Biol. 235:1098-1116. Skerra, A., M. L. Dreher, and G. Winter. 1991. Filter screening of antibody Fab fragments secreted from individual bacterial colonies: Specific detection of antigen binding with a two-membrane system. Anal. Biochem. 196:151-155. Soderlund, E., A. C. Simonsson, and C. A. K. Borrebaeck. 1992. Phage display technology in antibody engineering: Design of phagemid vectors and in vitro maturation systems. Immunol. Revs. 130:109-124. Taki, S., M. Meiering, and K. Rajewsky. 1993. Targeted insertion of a variable region gene into the immunoglobulin heavy chain locus. Science 262:1268-1270. Tsumoto, K., Y. Nakaoki, Y. Ueda, K. Ogasahara, K. Yutani, K. Watanabe, and I. Kumagai. 1994. Effect of the order of antibody variable regions on the expression of the single-chain HyHELlO Fv fragment in E. coli and the thermodynamic analysis of its antigen-binding properties. Biochem. Biophys. Res. Comm. 201(2):546-551. Waterhouse, P., A. D. Griffiths, K. S. Johnson, and G. Winter. 1993. Combinatorial infection and in vivo recombination: A strategy for making large phage antibody repertoires. Nucl. Acids Res. 21(9):2265-2266. Whitlow, M., and D. Filpula. 1991. Single-chain Fv proteins and their fusion proteins. METHODS: A Companion to Methods in Enzymology 2(2):97-105. Whitlow, M., B. A. Bell, SL. Feng, D. Filpula, K. D. Hardman, S. L. Hubert, M. L. Rollence, J. F. Wood, M. E. Schott, D. E. Milenic, T. Yokota, and J. Schlom. 1993. An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability. Protein Eng. 6(8):989-995. Wilson, I. A., and R. L. Stanfield. 1993. Antibody-antigen interactions. Curr. Opin. Struct. Biol. 3:113-118. Wu, XC , SC. Ng, R. I. Near, and SL. Wong. 1993. Efficient production of a functional single-chain antidigoxin antibody via an engineered Bacillus subtilis expression-secretion system. Bio/Technol. 11:71-76. Zou, YR., H. Gu, and K. Rajewsky. 1993. Generation of a mouse strain that produces immunoglobulin K chains with human constant regions. Science 262:1271-1274.